IDENTIFICATION AND MECHANISM STUDY OF THE GERMIN-LIKE PROTEINS FAMILY GENE OF HBGLP01 FROM RUBBER TREES

Abstract

Corynespora leaf fall disease (CLFD) is one of the most destructive leaf diseases in the rubber plantations. Cultivation of resistant clones developed from disease resistant germplasm is the most efficient way to control this disease. Identification of resistant genes is the basis for selecting germplasm resources of disease resistance to breed resistant varieties. From recent studies, we have cloned a member of the germin-like proteins family encoding HbGLP01 gene by cDNA amplified fragment length polymorphism technique. This gene was able to respond to pathogen infection in resistant germplasm, and had the potential of becoming a target gene in early molecular diagnosis of rubber tree germplasm. In this study, we propose to identify HbGLP01 gene(s) from complete genome sequence database of rubber trees, and analyze the genetic evolutionary relationships of the germin-like protein family genes. Its expression characteristics toward biotic stress, exogenous hormone treatment in the resistant/susceptible germplasm of rubber trees will be studied based on the analysis of tissue specific expression. These results will make clear of the roles of these important genes in resistance and defense signaling pathway in rubber trees.

Keywords: Hevea brasiliensis, Corynespora leaf fall disease, germin-like proteins, family encoding gene HbGLP01, resistant genes, rubber, molecular diagnosis, germplasm, genetic evolutionary relationships