ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL IAA (INDOLE ACETIC ACID) DARI RHIZOSFER TANAMAN KARET (Hevea brasiliensis Müll. Arg.)
DOI:
https://doi.org/10.22302/ppk.jpk.v42i2.879Keywords:
bakteri, Hevea brasiliensis, indole acetic acid (IAA), isolasi, rhizosferAbstract
Indole Acetic Acid (IAA) merupakan hormon yang penting untuk memacu pertumbuhan akar tanaman. Penelitian ini bertujuan memperoleh bakteri dari tanah sekitar perakaran tanaman karet yang mampu menghasilkan hormon IAA. Bakteri yang diisolasi dari tanah di sekitar perakaran tanaman karet kemudian di uji respons hipersensitifitas (HR), kemampuan berbiak serta tumbuh dan kemudian isolate terpilih dikarakterisasi. Hasil isolasi, diperoleh 30 isolate bakteri yang berbeda, yang terdiri dari 9 bakteri patogen dan 21 bakteri non-patogen. Seleksi kemampuan menghasilkan IAA menunjukkan bahwa dari isolate non patogen hanya lima isolate yang mampu menghasilkan IAA. Kemampuan menghasilkan IAA tertinggi dan terendah masing-masing isolate setelah inkubasi 72 jam adalah isolate B12 yakni sebesar 37,379 ppm dan isolate B17 sebesar 14,707 ppm. Bagaimanapun juga pengujian kemampuan isolate terseleksi menghasilkan IAA dalam interaksinya dengan tanaman karet perlu diuji.
References
Astuti, R. P. 2007. Rhizobakteria Bacillus sp Asal Tanah Rhizosfer Kedelai yang Berpotensi Sebagai Pemacu Pertumbuhan Tanaman. Thesis:Sekolah Pascasarjana Institut Pertanian Bogor. Bogor : IPB.
Butarbutar, R., H. Marwan, dan S. Mulyati. 2018. Eksplorasi Bacillus spp. dari Rhizosfer Tanaman Karet (Hevea brasiliensis) dan Potensinya Sebagai Agens Hayati Jamur Akar Putih (Rigidoporus sp.). Jurnal Agroecotania. 1(2):31-41.
Coronado, R.A.F. F. R. Q. Figueroa, L. M. G. Perez, E. R. Chavez, J. M. Torres, and I. E. M. Mendoza. 2014. IAA-Producing Rhizobacteria from Chickpea (Cicer arietinum L.) Induce Change in Root Architecture and Increase Root Biomass. Canadian Journal if Microbiology. 60(10):639-648.
Davies, R., A. Di Sacco, and R. Newton. 2015. Germination Testing: Procedures and Evaluation. Royal Botanic Garden. 1-4.
Dirjenbun. 2022. Statistik Perkebunan Unggulan Nasional 2020-2022. Jakarta:Direktorat Jenderal Perkebunan.
Gardner, T., V. Acosta-Martinez, Z. Senwo, and S. E. Dowd. 2011. Soil Rhizosphere Microbial Communities and Enzyme Activities Under Organic Farming in Alabama. Diversity. 3(3):308-328.
Goswami, D., J. N. Thakker, and P. C. Dhandhukia. 2015. Simultaneous Detection and Qualification of Indole-3-Acetic Acid (IAA) and Indole-3-Butyric Acid (IBA) Production by Rhizobacteria from I-Tryptophan (Trp) Using HPTLC. Journal of Microbiological Methods. 110:7-14.
Haris, N., Sumaryono, Siswanto, Sumarmadji, and C. Marc-Pillippe. 2010 Microcuttig of Hevea Rubber Genotype 78 and 91. China Agriculture Press. pp. 311-316.
Hidayati, U. 2014. Potensi Bakteri Endofit Asal Tanaman Karet Sebagai Pemacu Petumbuhan Bibit Batang Bawah Tanaman Karet (Hevea brasiliensis Müll. Arg.). Disertasi. Institut Pertanian Bogor (IPB).
Khaeruni, A. R., B. Tjahjono, A. Suwanto, dan M. S. Sinaga. 2008. Virulensi Sejumlah Isolate Xanthomonas axonopodis pv glycines Asal Edamame pada Tiga Varietas Kedelai. Jurnal HPT Tropika. 8(1):39-46.
Klement, Z. and R. N. Goodman. 1967 The Hypersensitive Reaction on Infection by Bacterial Plant Pathogens. Annual Review of Phytopathology. 5(1);17-44.
Krol, P., J. Adamska, and E. Kepezynska. 2014. Enhancement of Festuca rubra L. Germination and Seedling Growth by Seed Treatment with Pathogenic Agrobacterium rhizogenes. Acta Physiologiae Plantarum. 36:3263-3274.
Lambreacht, M., Y. Okon, A. V. Broek, and J. Vanderleyden. 2000. Indole-3-Acetic Acid: a Reciprocal Signalling Molecule Bacteria-Plant Interaction. Trends in Microbiology. 8(7):298-300.
Li, S. W. L. Xue, S. Xu, H. Feng, and L. An. 2009. Mediators, Genes, and Signaling in Addventitious Rooting. The Botanical Review. 75:230-247.
Liwarska-Bizukojc. 2022. Phytotoxicity Assessment of Biodegradable and Non-Biodegradable Plastics Using Seed Germination and Early Growth Tests. Chemosphere. 289:1-8.
Pal, A. K., S. Mandal, and C. Sengupta. 2019. Exploitation of IAA Producing PGPR on Mustard (Brassica nigra L.) Seedling Growth Under Cadmium Stress Condition in Comparison with Exogenous IAA Application. Plant Science Today. 6(1):22-30.
Rahma, H., A, Zainal, M. Surahman, M. S. Sinaga, dan Giyanto. 2014. Potensi Bakteri Endofit dalam Menekan Penyakit Layu Stewart (Pantoea Stewartii subsp. Stewartii) pada Tanaman Jagung. Jurnal HPT Tropika. 14(2):121-137.
Rini, I. A., I. Oktaviani, M. Asril, R. Agustin, dan F. K. Frima. 2020. Isolasi dan Karakterisasi Bakteri Penghasil IAA (Indole Acetic Acid) dari Rhizosfer Tanaman Akasia (Acacia mangium). Agro Bali: Agricultural Journal. 3(2):210-219.
Sukmawati, S., A. Adnyana, D. N. Suprapta, M. Proborini, P. Soni, and P.G. Adinurani. 2021. Multiplication Arbuscular Mycorrhizal Fungi in Corn (Zea mays L.) with Pots Culture at Greenhouse. E3S Web of Conferences. 226:1-10.
Talaiekhozani, A. S. Alaee, and M. Ponraj. 2015. Guidelines for Quick Application of Biochemical Test to Identify Unknown Bacteria. Account of Biotechnology Research. 2(2):65-82.
Ulya, T. H., R. Rogomulyo, dan L. Admojo. 2019. Pengaruh Konsentrasi IBA Terhadap Pertumbuhan Akar Dua Fase Warna Batang pada Stek Batang Bawah Karet (Hevea brasiliensis Muell. Arg.). Jurnal Penelitian Karet. 37(2):151-162.
Wei, X., J. Chen, C. Zhang, H. Liu, X. Zheng, and J. Mu. 2020. Ericoid Mycirrhizal Fungus Enhances Microcutting Rooting of Rhododendron fortunei and Subsequent Growth. Horticulture Research. 7:1-11.
Yousif, E., B. Abdullah, H. Ibraheem, J. Salimon, and N. Salih. 2013. Rubber Seed Oil Properties, Authentication, and Quality Assessment Using (Chloroform: Methanol) as Solvent. Journal of Al-Nahrain University. 16(3):1-6.
Zhang, C., H. Chen, R.R. Zhuang, Y. T. Chen, Y. Deng, T. C. Cai, S. Y. Wang, Q. Z. Liu, R. H. Tang, S. H. Shan, R. L. Pan, L. S. Chen, and W. J. Zhuang. 2019. Overexpression of The Peanut CLAVATA1- like Leucine-Rich Repeat Receptor-Like Kinase AhRLK1 Confers Increased Resistance to Bacterial with in Tobacco. Journal of Experimental Botany. 70(19):5407-5421.
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